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KMID : 0377519900150020135
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Chung-Ang Journal of Medicine
1990 Volume.15 No. 2 p.135 ~ p.153
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Effects of Candmium on Htdrogen Peroxide Production, Catalase and Na^(+), K^(+) -ATPase Activities of Rat Kidney and Protective Effects of Ascorbate, Diethyldithiocar-bamate(DDTC), Glutathione and α-Tocopherol on Them
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Abstract
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The effects of cadmium and protective effects of ^ascorbate, diethyldithiocarbamate (DDTC), glutathione and a-tocopherol on hydrogen peroxide production, Catalase and Na´, K+-ATPase activities in kidney of rats acutely intoxicated by cadmium were investigated. A single dose of´ 5 mg/kg CdClz were given to all Sprague-Dawley male rats by intraperitoneal injection.
xk In addition, 1 mM/kg ascorbate in Cd + ascorbate-treated group, 500 mg/kg DDTC in
Cd + DDTC-treated group, 1 mM/kg glutathione in Cd + glutathione-treated group and 0.5` mM/kg
n a-tocopherol in Cd + a-tocopherol-treated group were given intraperitoneally 60 min after the cadmium treatment. The saline-treated group was treated with saline only. Rats of each group were sacrificed by decapitation 48 hours after the cadmium treatment.
The cadmium concentration in kidney increased with that in blood both in saline and experimental groups. Average cadmium concentrations in blood and kidney of Cd-treated rats were much higher than those in saline-treated group, but no differences were found in average cadmium concentrations in blood and kidney among Cd-treated, Cd + ascorbate -treated, Cd + DDTC-treated, Cd + glutathione-treated and Cd + a-tocopherol-treated groups.
In Cd-treated group, hydrogen peroxide production and Catalase activity of kidney homogenates was increased but Na+, K+-ATPase activity was decreased. Glutathione inhibited cadmium-induced hydrogen peroxide production most effectively. Ascorbate, DDTC and a-tocopherol also inhibited cadmium-induced hydrogen peroxide production. Catalase activity was inhibited in ascorbate-and glutathione-treated groups more than that in Cd-treated group, and no difference was found in Catalase activities among Cd + DDTC-treated and Cd + a-tocopherol-treated groups. Inhibition of Na+, K+-ATPase activity induced by cadmium was diminished by simultaneous administration of ascorbate, DDTC, glutathione or cr-tocopherol.
In normal and experimental groups, hydrogen peroxide production increased with increasincadmium concentrations and the Na´, K´-ATPase activity decreased with increasing hydrogen peroxide productions These results suggest that inhibition of Na´, K+-ATPase activity caused by hydrogen peroxide in cadmium intoxicated rats may play a part in cadmium induced renal functional injury Ascorbate, DDTC, glutathione and a-tocopherol exhibit protective effects against cadmium mediated inhibition of Na´, K´-ATPase activity and these effects brought by inhibition of cadmium-induced hydrogen peroxide production.
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